The Human Integrin Alpha 11 (ITGA11) ELISA Kit is a cutting-edge tool specifically designed for the precise measurement of ITGA11 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an indispensable asset for various research purposes.Integrin Alpha 11 is a key protein involved in cell adhesion and signaling, playing a crucial role in processes like cell migration, tissue repair, and fibrosis.
Its dysregulation has been associated with various pathological conditions, including cancer, fibrotic diseases, and inflammatory disorders, highlighting the significance of ITGA11 as a potential biomarker for disease diagnostics and therapeutic interventions.Don't miss out on the opportunity to explore the functions and implications of Integrin Alpha 11 with our top-quality ELISA Kit, empowering you to unravel new insights and advance your research in the field of cellular biology and disease mechanisms.
Product Name:
Human Integrin Alpha 11 (ITGa11) ELISA Kit
Product Code:
HUDL01562
Size:
96 Assays
Synonyms:
BYE1, DATF1, DIDO2, DIDO3, DIO1, Death Associated Transcription Factor 1
Detection Method:
sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
