Human Insulin Receptor Pathway Phosphorylation Array (9 targets) (SARB0053)
- SKU:
- SARB0053
- Product Type:
- Phosphorylation Array
- Reactivity:
- Human
- Applications:
- Multiplex Array
Description
Human Insulin Receptor Pathway Phosphorylation Array (9 targets) (SARB0053)
The Human Insulin Receptor Pathway Phosphorylation Array (SARB0053) is a powerful tool for studying the phosphorylation status of 9 key targets involved in the insulin receptor pathway. This array allows for the simultaneous detection and quantification of phosphorylation levels, providing valuable insights into the signaling events that regulate insulin receptor activity.With a focus on the insulin receptor pathway, this array is ideal for researchers interested in studying metabolic disorders, diabetes, and related conditions. By examining the phosphorylation status of key signaling molecules in this pathway, researchers can gain a better understanding of the molecular mechanisms underlying insulin signaling and its impact on cellular function.The Human Insulin Receptor Pathway Phosphorylation Array offers high specificity and sensitivity, allowing for the detection of subtle changes in phosphorylation levels.
This array is compatible with various sample types, including cell lysates and tissue homogenates, making it a versatile tool for both basic research and drug discovery.Overall, the Human Insulin Receptor Pathway Phosphorylation Array is a valuable resource for researchers seeking to unravel the complexities of insulin signaling and its role in disease. Its comprehensive coverage of key phosphorylation events in the insulin receptor pathway makes it a valuable asset for studies aiming to elucidate the mechanisms underlying insulin resistance, metabolic dysfunction, and related pathologies.
Product Sku: | SARB0053 |
Size: | 2, 4, or 8 |
Species Detected: | Human |
Number of Targets Detected: | 9 |
Gene Symbols: | EIF4E, FOXO3, IGF1R, INPP5D, INS, IRS1, PTPN11, SHC1, STK11 |
Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
Design Principle: | Sandwich-based |
Method of Detection: | Chemiluminescence |
Pathway: | Akt Signaling / IGF Signaling / mTOR Signaling / PI3K-AKT Signaling |
Quantitative/Semi-Quantitative: | Semi-Quantitative |
Solid Support: | Membrane |
Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
Hover over target for more information | ||||
---|---|---|---|---|
eIF-4E (P-Ser209)
| FOXO-3 (P-Ser413)
| IGF-1 R (P-Tyr1165)
| Insulin (P-Tyr1189)
| IRS-1 (P-Ser318)
|
LKB1 (P-Ser428)
| SHC (P-Tyr427)
| SHIP1 (P-Tyr1020)
| SHP-2 (P-Ser542)
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Insulin Receptor Phosphorylation Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1. | Block membranes |
2. | Incubate with Sample |
3. | Incubate with Detection Antibody Cocktail |
4. | Incubate with HRP-Conjugated anti-IgG |
5. | Incubate with Detection Buffers |
6. | Image with chemiluminescent imaging system |
7. | Perform densitometry and analysis |