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Human Insulin Receptor (ISR) ELISA Kit

SKU:
HUDL01559
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P06213
Sensitivity:
0.58ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
CD220,INSR,HHF5,IR
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Insulin Receptor (ISR) ELISA Kit

The Human Insulin Receptor (ISR) ELISA Kit is a highly reliable and accurate tool for detecting levels of insulin receptor in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.The insulin receptor plays a critical role in regulating glucose metabolism, cell growth, and differentiation. Dysregulation of insulin signaling is associated with various metabolic disorders, including diabetes and obesity.

By accurately measuring insulin receptor levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.With its robust performance and versatile applications, the Human Insulin Receptor (ISR) ELISA Kit is an indispensable tool for studying insulin receptor biology and its implications in human health and disease.

Product Name:Human Insulin Receptor (ISR) ELISA Kit
Product Code:HUDL01559
Size:96 Assays
Target:ISR
Synonyms:CD220, INSR, HHF5, IR
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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