Human Insulin Like Growth Factor Binding Protein 5 (IGFBP5) ELISA Kit
The Human Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA Kit is specifically designed for the precise measurement of IGFBP5 levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and dependable results, making it an excellent choice for various research applications.IGFBP5 is a key protein involved in regulating the activity of insulin-like growth factors, playing a crucial role in cell growth, proliferation, and differentiation.
Dysregulation of IGFBP5 has been linked to various diseases such as cancer, diabetes, and growth disorders, highlighting its importance as a potential biomarker for disease progression and therapeutic development.Overall, the Human IGFBP5 ELISA Kit provides researchers with a valuable tool for studying the role of IGFBP5 in physiological and pathological processes, opening up new possibilities for advancing our understanding of disease mechanisms and identifying potential therapeutic targets.
Product Name:
Human Insulin Like Growth Factor Binding Protein 5 (IGFBP5) ELISA Kit
Product Code:
HUDL01452
Size:
96 Assays
Target:
IGFBP5
Synonyms:
IBP5
Detection Method:
Sandwich
Range:
6.25-400ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
