Human Insulin Like Growth Factor Binding Protein 4 (IGFBP4) ELISA Kit
The Human Insulin-like Growth Factor Binding Protein 4 (IGFBP4) ELISA Kit is specifically designed for the quantitative detection of IGFBP4 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.IGFBP4 plays a crucial role in regulating the bioavailability and activity of insulin-like growth factors, which are essential for cell growth and development. Abnormal IGFBP4 levels have been associated with various diseases, including cancer, diabetes, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With its reliable performance and ease of use, the Human IGFBP4 ELISA Kit is a valuable tool for researchers and clinicians working in the fields of endocrinology, oncology, and cardiovascular research. Order yours today to advance your research efforts and gain valuable insights into IGFBP4 biology and its role in human health and disease.
Product Name:
Human Insulin Like Growth Factor Binding Protein 4 (IGFBP4) ELISA Kit
Product Code:
HUDL01451
Size:
96 Assays
Target:
IGFBP4
Synonyms:
BP4, HT29-IGFBP, IBP4
Detection Method:
Sandwich
Range:
3.125-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
