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Human Insulin Like Growth Factor 2 Receptor (IGF2R) ELISA Kit

SKU:
HUDL01446
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11717
Sensitivity:
0.244ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
CD222,IGF2-R,CIMPR,M6P-R,MPRI,Cation-Independent Mannose-6 Phosphate Receptor,M6P/IGF2 receptor,300 kDa mannose 6-phosphate receptor
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Human Insulin Like Growth Factor 2 Receptor (IGF2R) ELISA Kit

The Human Insulin-like Growth Factor 2 Receptor (IGF2R) ELISA Kit is a powerful tool for the quantitative measurement of IGF2R levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, allowing for accurate and reliable results that are crucial for various research applications.IGF2R is a vital receptor involved in the regulation of insulin-like growth factor 2 (IGF2) signaling, playing a significant role in cell growth, proliferation, and differentiation.

Dysregulation of IGF2R has been linked to various diseases, including cancer and metabolic disorders, making it a key target for understanding disease mechanisms and potential therapeutic interventions.With its user-friendly format and efficient performance, the Human IGF2R ELISA Kit is an essential tool for researchers studying IGF2R biology and its implications in health and disease. Get precise and insightful data with this advanced ELISA kit and unlock new possibilities in your research endeavors.

Product Name:Human Insulin Like Growth Factor 2 Receptor (IGF2R) ELISA Kit
Product Code:HUDL01446
Size:96 Assays
Target:IGF2R
Synonyms:CD222, IGF2-R, CIMPR, M6P-R, MPRI, Cation-Independent Mannose-6 Phosphate Receptor, M6P/IGF2 receptor, 300 kDa mannose 6-phosphate receptor
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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