Human Insulin Like Growth Factor 1 Receptor (IGF1R) ELISA Kit
The Human Insulin-Like Growth Factor 1 Receptor (IGF-1R) ELISA Kit is a powerful tool for accurate quantification of IGF-1R levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a wide range of research applications.IGF-1R is a key receptor involved in mediating the effects of insulin-like growth factor 1 (IGF-1) signaling, which plays a crucial role in regulating cell growth, proliferation, and survival.
Dysregulation of IGF-1R signaling has been implicated in various diseases including cancer, diabetes, and cardiovascular disorders, making it a valuable target for therapeutic intervention.By using the Human IGF-1R ELISA Kit, researchers can accurately measure IGF-1R levels in biological samples, allowing for in-depth investigation of IGF-1R signaling pathways and potential therapeutic strategies targeting this receptor. Trust in the reliability and precision of this ELISA kit for your research needs.
Product Name:
Human Insulin Like Growth Factor 1 Receptor (IGF1R) ELISA Kit
Product Code:
HUDL01443
Size:
96 Assays
Target:
IGF1R
Synonyms:
CD221, IGF1-R, IGFIR, JTK13
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
