Description
Product Name: | Human Insulin IgG Autoantibodies ELISA Kit |
SKU: | HDES0073 |
Size: | 96T |
Target: | INS-Ab |
Detection Method: | Indirect ELISA |
Reactivity: | Human |
Result Type: | Qualitative: Sensitivity 98.2%, Specificity 99.7% |
Sample Type: | Serum |
Detection Wavelength: | 450/630nm |
Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Indirect-ELISA as the method to detect the Insulin (INS) IgG antibody in human serum. The micro ELISA plate provided in this kit has been pre-coated with purified recombinant INS antigen. Samples are added to the ELISA Microtiter plate wells and the INS-Ab in which will combine with the pre-coated antigen to form antigen-antibody compound. Free components are washed away. The HRP conjugate is added to each well and react with the compound to form antigen- antibody-HRP conjugate compound. The substrate reagent is added to initiate the color developing reaction. The presence of INS-Ab can be determined according to the OD value after colorimetric assay with the Micro-plate reader.