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Human Insulin Degrading Enzyme (IDE) ELISA Kit

SKU:
HUDL01425
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P14735
Sensitivity:
22.7pg/mL
Range:
62.5-4000pg/mL
ELISA Type:
Sandwich
Synonyms:
Insulysin,Insulin Protease,Abeta-degrading protease,Insulinase
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Insulin Degrading Enzyme (IDE) ELISA Kit

The Human Insulin Degrading Enzyme (IDE) ELISA Kit is specifically designed for the precise quantification of IDE levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it suitable for a variety of research purposes.IDE is an important enzyme responsible for the degradation of insulin, and abnormalities in its function have been linked to various metabolic disorders such as diabetes.

By measuring IDE levels, researchers can gain valuable insights into insulin metabolism and its role in metabolic diseases, paving the way for the development of new therapeutic strategies.Overall, the Human IDE ELISA Kit is a valuable tool for studying the role of IDE in insulin metabolism and metabolic disorders, offering researchers a reliable and efficient method for analyzing IDE levels in biological samples.

Product Name:Human Insulin Degrading Enzyme (IDE) ELISA Kit
Product Code:HUDL01425
Size:96 Assays
Target:IDE
Synonyms:Insulysin, Insulin Protease, Abeta-degrading protease, Insulinase
Detection Method:Sandwich
Range:62.5-4,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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