The Human INSL3 (Insulin-like Protein 3) ELISA Kit is specifically designed for the accurate detection of INSL3 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.INSL3 is a key protein that acts as a biomarker for various physiological processes, including reproductive development and function. It plays a crucial role in testicular descent during fetal development and is also involved in the regulation of testosterone production in adults.
Understanding the role of INSL3 is essential for studying reproductive disorders and developing potential therapies.With the Human INSL3 ELISA Kit, researchers can confidently measure and analyze INSL3 levels with confidence, furthering our knowledge of this important protein and its implications in various biological processes.
Product Name:
Human INSL3 (Insulin Like Protein 3) ELISA Kit
SKU:
HUES01620
Target:
Human INSL3 (Insulin Like Protein 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human INSL3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human INSL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INSL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human INSL3. You can calculate the concentration of Human INSL3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
91-104
99-114
Average (%)
105
96
105
1:4
Range (%)
91-103
80-91
85-98
Average (%)
96
86
91
1:8
Range (%)
87-101
86-97
83-95
Average (%)
93
91
89
1:16
Range (%)
92-104
80-91
84-95
Average (%)
98
86
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-109
100
EDTA plasma (n=5)
94-107
99
Cell culture media (n=5)
94-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.05
4.92
0.44
1.06
4.5
Standard deviation
0.03
0.06
0.17
0.03
0.05
0.22
C V (%)
6.38
5.71
3.46
6.82
4.72
4.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human INSL3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human INSL3 in samples. No significant cross-reactivity or interference between Human INSL3 and analogues was observed.
