The INS ELISA Kit is designed for the quantitative detection of Insulin (INS) levels in various human biological samples. Being a vital hormone, insulin is key for diverse cellular functions, including glucose metabolism, cellular growth, and differentiation. It plays an important role in the body's metabolic response by regulating the glucose levels in the blood, and imbalances often lead to critical health conditions like diabetes. Accurate measurement of insulin is crucial for understanding its involvement in metabolic processes and can provide key insights for strategising suitable therapeutic interventions.
Assay Genie's INS ELISA Kit offers outstanding sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under rigorous quality control standards, this kit provides formidable performance and is user-friendly, making it an excellent choice for both research and clinical applications. Trust Assay Genie's INS ELISA Kit for accurate and dependable quantification of this vital biomarker in your studies.
Product Name:
Human INS (Insulin) ELISA Kit
SKU:
AEES00036
Target:
Human INS (Insulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 μIU/mL
Detection range:
0.78-50 μIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human INS. You can calculate the concentration of Human INS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-95
98-110
96-108
Average (%)
90
104
102
1:4
Range (%)
95-105
88-98
86-95
Average (%)
99
95
92
1:8
Range (%)
98-108
93-103
98-111
Average (%)
105
100
104
1:16
Range (%)
93-106
82-95
100-112
Average (%)
99
90
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
94
EDTA plasma (n=5)
96-108
101
Cell culture media (n=5)
94-106
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.38
5.03
23.63
2.21
6.19
21.47
Standard deviation
0.13
0.25
1.25
0.11
0.29
0.97
C V (%)
5.46
4.97
5.29
4.98
4.68
4.52
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human INS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human INS in samples. No significant cross-reactivity or interference between Human INS and analogues was observed.
