The Human INHB (Inhibin B) CLIA Kit is a cutting-edge assay designed for the precise measurement of inhibin B levels in human samples such as serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers consistent and accurate results, making it an invaluable tool for various research applications. Inhibin B is a vital hormone that plays a crucial role in reproductive health, particularly in regulating follicle-stimulating hormone (FSH) levels and aiding in the maturation of ovarian follicles. Abnormal levels of inhibin B have been linked to infertility, polycystic ovary syndrome (PCOS), and other reproductive disorders, making it a key biomarker for studying and managing these conditions.
By utilizing the Human INHB CLIA Kit, researchers can gain valuable insights into the role of inhibin B in reproductive health and potentially identify novel therapeutic targets for improving fertility and treating related disorders. Its ease of use and reliable performance make it an essential tool for advancing our understanding of reproductive physiology and developing innovative treatment strategies.
Product Name:
Human INHB (Inhibin B) CLIA Kit
SKU:
HUES00227
Target:
Human INHB (Inhibin B)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human INHB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human INHB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INHB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human INHB. You can calculate the concentration of Human INHB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
94-107
90-104
Average (%)
105
101
98
1:4
Range (%)
100-114
99-117
95-111
Average (%)
106
107
103
1:8
Range (%)
92-105
95-110
95-108
Average (%)
98
102
100
1:16
Range (%)
85-99
94-109
98-112
Average (%)
91
100
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
106
EDTA plasma (n=5)
91-105
97
Cell culture media (n=5)
93-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.37
71.19
229.07
24.82
69.8
218.91
Standard deviation
3.13
7.92
19.59
2.5
7.07
22.29
C V (%)
11.44
11.13
8.55
10.07
10.13
10.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human INHB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human INHB in samples. No significant cross-reactivity or interference between Human INHB and analogues was observed.
