Human Inflammation Array (20 targets) (SARB0051)
- SKU:
- SARB0051
- Product Type:
- Protein Array
- Analytes:
- ICAM-1 (CD54)
- IL-6 R
- IL-15
- IL-16
- IL-17A
- IP-10 (CXCL10)
- MCP-1 (CCL2)
- MCP-2 (CCL8)
- M-CSF
- MIG (CXCL9)
- MIP-1 alpha (CCL3)
- MIP-1 beta (CCL4)
- MIP-1 delta (CCL15)
- RANTES (CCL5)
- TGF beta 1
- TNF alpha
- TNF beta (TNFSF1B)
- TNF RI (TNFRSF1A)
- TNF RII (TNFRSF1B)
- PDGF-BB
- Reactivity:
- Human
- Applications:
- Multiplex Array
Frequently bought together:
Description
system_update_altDatasheet
| Product Sku: | SARB0051 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 20 |
| Gene Symbols: | CCL15, CCL2, CCL3, CCL4, CCL5, CCL8, CSF1, CXCL10, CXCL9, ICAM1|IL15, IL16, IL17A, IL6R, LTA, PDGFB, TGFB1, TNF, TNFRSF1A, TNFRSF1B |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | MAPK Signaling / NFkB Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
ICAM-1 (CD54)
| IL-6 R
| IL-15
| IL-16
| IL-17A
|
IP-10 (CXCL10)
| MCP-1 (CCL2)
| MCP-2 (CCL8)
| M-CSF
| MIG (CXCL9)
|
MIP-1 alpha (CCL3)
| MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
| RANTES (CCL5)
| TGF beta 1
|
TNF alpha
| TNF beta (TNFSF1B)
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
| PDGF-BB
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Inflammation Antibody Array C2 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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