Human Immune Receptor Expressed On Myeloid Cells 1 (IREM1) ELISA Kit
The Human Immune Receptor Expressed on Myeloid Cells 1 (IREM1) ELISA Kit from Assay Genie is a powerful tool for the accurate measurement of IREM1 levels in human samples. This kit is specifically designed for use with serum, plasma, and cell culture supernatants, providing researchers with a versatile and reliable solution for their studies.IREM1 is an important immune receptor that plays a key role in the regulation of myeloid cell function. Dysregulation of IREM1 has been implicated in various diseases, including autoimmune disorders and inflammatory conditions.
By accurately measuring IREM1 levels, researchers can gain valuable insights into the immune response and potentially identify new therapeutic targets for these diseases.With its high sensitivity and specificity, the IREM1 ELISA Kit ensures consistent and reproducible results, allowing researchers to confidently analyze their samples. Whether investigating the role of IREM1 in immune responses, inflammation, or other physiological processes, this kit provides a valuable tool for advancing research in the field of immunology and beyond.
Product Name:
Human Immune Receptor Expressed On Myeloid Cells 1 (IREM1) ELISA Kit
Product Code:
HUDL01550
Size:
96 Assays
Target:
IREM1
Synonyms:
CD300f, CD300LF, NKIR, IGSF13, CLM1, CD300 Antigen Like Family Member F, CMRF35-Like Molecule 1, Immunoglobulin superfamily member 13, NK inhibitory receptor
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
