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Human Immune Checkpoint Array (12 targets) (SARB0049)

SKU:
SARB0049
Product Type:
Protein Array
Analytes:
4-1BB (TNFRSF9/CD137)
B7-H2 (ICOS Ligand)
B7-H3 (CD276)
B7-H4
CD200
HVEM (TNFRSF14/CD270)
CD27 Ligand (TNFSF7)
CD80 (B7-1)
CD86 (B7-2)
OX40 Ligand (TNFSF4)
B7-H1 (CD274/PD-L1)
PD-L2 (B7-DC)
Reactivity:
Human
Applications:
Multiplex Array
$575 - $1,115
Frequently bought together:

Description

system_update_altDatasheet

Human Immune Checkpoint Array (12 targets) (SARB0049)

The Human Immune Checkpoint Array - 12 Targets (SARB0049) is a comprehensive tool for studying immune checkpoint molecules in human samples. This array includes 12 key targets involved in immune regulation, allowing researchers to simultaneously analyze multiple proteins in a single experiment. Each target on the array is carefully selected based on its role in modulating immune responses, making it ideal for investigating pathways involved in immune tolerance, autoimmunity, and anti-tumor immunity. The array provides a high level of sensitivity and specificity, enabling precise quantification of protein expression levels in different biological samples.With this Human Immune Checkpoint Array, researchers can gain valuable insights into the complex interactions between immune cells and their microenvironment.

By analyzing the expression patterns of immune checkpoint molecules, researchers can identify potential biomarkers for disease diagnosis and prognosis, as well as potential targets for therapeutic intervention.Overall, the Human Immune Checkpoint Array - 12 Targets (SARB0049) offers a powerful tool for advancing research in immunology, oncology, and drug development. Its comprehensive design and reliable performance make it a valuable resource for scientists studying immune checkpoint pathways and their implications in various disease states.

Product Sku:SARB0049
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:12
Gene Symbols:CD200, CD274, CD276, CD70, CD80, CD86, ICOSLG, PDCD1LG2, TNFRSF14, TNFRSF9|TNFSF4, VTCN1
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
4-1BB (TNFRSF9/CD137)
  • Gene Symbol: TNFRSF9 CD137 ILA
  • Gene ID: 3604
  • Accession #: Q07011
B7-H2 (ICOS Ligand)
  • Gene Symbol: ICOSLG B7H2 B7RP1 ICOSL KIAA0653
  • Gene ID: 102723996; 23308
  • Accession #: O75144
B7-H3 (CD276)
  • Gene Symbol: CD276 B7H3 PSEC0249 UNQ309/PRO352
  • Gene ID: 80381
  • Accession #: Q5ZPR3
B7-H4
  • Gene Symbol: VTCN1 B7H4 UNQ659/PRO1291
  • Gene ID: 79679
  • Accession #: Q7Z7D3
CD200
  • Gene Symbol: CD200 MOX1 MOX2 My033
  • Gene ID: 4345
  • Accession #: P41217
HVEM (TNFRSF14/CD270)
  • Gene Symbol: TNFRSF14 HVEA HVEM UNQ329/PRO509
  • Gene ID: 8764
  • Accession #: Q92956
CD27 Ligand (TNFSF7)
  • Gene Symbol: CD70 CD27L CD27LG TNFSF7
  • Gene ID: 970
  • Accession #: P32970
CD80 (B7-1)
  • Gene Symbol: CD80 CD28LG CD28LG1 LAB7
  • Gene ID: 941
  • Accession #: P33681
CD86 (B7-2)
  • Gene Symbol: CD86 CD28LG2
  • Gene ID: 942
  • Accession #: P42081
OX40 Ligand (TNFSF4)
  • Gene Symbol: TNFSF4 TXGP1
  • Gene ID: 7292
  • Accession #: P23510
B7-H1 (CD274/PD-L1)
  • Gene Symbol: CD274 B7H1 PDCD1L1 PDCD1LG1 PDL1
  • Gene ID: 29126
  • Accession #: Q9NZQ7
PD-L2 (B7-DC)
  • Gene Symbol: PDCD1LG2 B7DC CD273 PDCD1L2 PDL2
  • Gene ID: 80380
  • Accession #: Q9BQ51
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Immune Checkpoint C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis

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