The Human IL4I1 (Interleukin 4 Induced 1) ELISA Kit is specifically designed for the accurate measurement of IL4I1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity to ensure reliable and reproducible results, making it a valuable tool for diverse research applications. IL4I1 is a key enzyme that plays a critical role in modulating immune responses and inflammation. Its dysregulation has been linked to various immune-related disorders, making it a promising biomarker for understanding disease mechanisms and developing targeted therapies.
The Human IL4I1 ELISA Kit provides a simple and efficient way to quantify IL4I1 levels in human samples, allowing researchers to investigate its role in various biological processes and diseases. Get accurate and reliable results with this high-quality ELISA kit from Assay Genie.
Product Name:
Human IL-4I1 ELISA Kit
SKU:
HUES03170
Target:
Human IL-4I1
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL4I1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL4I1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL4I1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL4I1. You can calculate the concentration of Human IL4I1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
89-104
91-104
Average (%)
106
95
97
1:4
Range (%)
92-102
80-92
88-101
Average (%)
97
86
94
1:8
Range (%)
91-106
78-90
87-102
Average (%)
96
85
94
1:16
Range (%)
91-106
88-101
88-101
Average (%)
98
93
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
91
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.56
4.0
0.52
1.47
4.19
Standard deviation
0.03
0.07
0.15
0.03
0.06
0.2
C V (%)
6
4.49
3.75
5.77
4.08
4.77
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL4I1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL4I1 in samples. No significant cross-reactivity or interference between Human IL4I1 and analogues was observed.
