The Human IL2R-alpha CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of IL2R-alpha levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers dependable and reproducible results, making it suitable for various research applications.IL2R-alpha, also known as CD25, is a key protein involved in immune regulation and cell growth. It plays a vital role in conditions such as autoimmune diseases, inflammation, and cancer, making it a valuable biomarker for understanding these diseases and potentially developing targeted therapies.
Overall, the Human IL2R-alpha CLIA Kit from AssayGenie provides researchers with a reliable tool for studying the role of IL2R-alpha in various physiological and pathological processes, contributing to advancements in medical research and potential clinical applications.
Product Name:
Human IL-2R alpha CLIA Kit
SKU:
HUES00606
Target:
Human IL-2R alpha
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-2Rα/CD25. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-2Rα/CD25 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-2Rα/CD25, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-2Rα/CD25. You can calculate the concentration of Human IL-2Rα/CD25 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-118
89-102
91-103
Average (%)
107
94
97
1:4
Range (%)
90-108
96-109
84-98
Average (%)
98
103
91
1:8
Range (%)
101-117
89-100
95-111
Average (%)
108
95
102
1:16
Range (%)
88-101
93-108
102-114
Average (%)
95
99
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
98
EDTA plasma (n=5)
86-96
91
Cell culture media (n=5)
88-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.65
248.0
859.3
91.46
261.88
781.11
Standard deviation
11.22
21.13
68.06
11.88
20.79
57.8
C V (%)
11.15
8.52
7.92
12.99
7.94
7.4
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-2Rα/CD25 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-2Rα/CD25 in samples. No significant cross-reactivity or interference between Human IL-2Rα/CD25 and analogues was observed.
