The Human IL-23 (Interleukin-23) CLIA Kit is a powerful tool for detecting interleukin-23 levels in human samples such as serum and plasma. With exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it ideal for a variety of research applications.Interleukin-23 is a key cytokine that plays a crucial role in regulating immune responses, particularly in autoimmune diseases and inflammatory conditions. Dysregulation of IL-23 has been linked to various autoimmune disorders, making it a valuable biomarker for understanding the underlying mechanisms of these diseases and developing targeted therapies.
With the Human IL-23 CLIA Kit, researchers can confidently and efficiently measure interleukin-23 levels, gaining valuable insights into the immune system and its role in disease pathology. Trust in this kit for reliable results and impactful discoveries in the field of immunology and autoimmune research.
Product Name:
Human IL-23 CLIA Kit
SKU:
HUES00103
Target:
Human IL-23
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-23. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-23, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-23. You can calculate the concentration of Human IL-23 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-111
97-113
88-99
Average (%)
105
104
93
1:4
Range (%)
92-106
90-104
91-106
Average (%)
100
97
98
1:8
Range (%)
90-106
101-114
91-105
Average (%)
98
108
97
1:16
Range (%)
86-100
94-105
99-115
Average (%)
92
99
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
85-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.33
123.31
291.27
38.63
132.15
289.83
Standard deviation
4.02
10.62
24.0
4.3
13.39
28.87
C V (%)
9.97
8.61
8.24
11.13
10.13
9.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-23 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-23 in samples. No significant cross-reactivity or interference between Human IL-23 and analogues was observed.
