The IL-8 ELISA Kit is a specialized tool designed for the precise quantification of Interleukin-8 (IL-8) levels in human biological samples. IL-8, a pivotal chemokine, is crucial in orchestrating inflammatory responses, neutrophil activation, and immune regulation. Its diverse functions in cell migration and host defense make accurate measurement of IL-8 indispensable for unraveling its role in various inflammatory conditions, immune disorders, and disease processes. Monitoring IL-8 levels using this kit provides essential insights for understanding immune responses and developing targeted therapeutic strategies.
Our IL-8 ELISA Kit boasts exceptional sensitivity and specificity, ensuring reliable and reproducible results critical for both research and clinical applications. Manufactured under stringent quality control standards, this kit guarantees robust performance and ease of use, offering a reliable method for the accurate quantification of IL-8. You can trust Assay Genie's IL-8 ELISA Kit for precise and dependable measurements of this key biomarker in your studies.
Product Name:
Human IL-8 ELISA Kit
SKU:
AEES00047
Target:
Human IL-8
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-8. You can calculate the concentration of Human IL-8 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
93-105
102-113
Average (%)
102
101
105
1:4
Range (%)
93-107
92-101
99-111
Average (%)
99
95
105
1:8
Range (%)
87-97
90-103
93-103
Average (%)
92
95
97
1:16
Range (%)
93-104
94-110
91-102
Average (%)
97
102
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
94
EDTA plasma (n=5)
95-102
98
Cell culture media (n=5)
98-106
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.34
60.67
245.62
24.62
56.15
233.52
Standard deviation
1.21
2.8
12.26
1.39
2.8
9.69
C V (%)
5.95
4.62
4.99
5.65
4.99
4.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-8 in samples. No significant cross-reactivity or interference between Human IL-8 and analogues was observed.
