Human IL 2 Recombinant Protein (RPPB0489)
- SKU:
- RPPB0489
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P60568
- Research Area:
- Cytokines
Description
| Product Name: | Human IL 2 Recombinant Protein |
| Product Code: | RPPB0489 |
| Size: | 10µg |
| Species: | Human |
| Target: | IL 2 |
| Synonyms: | Interleukin-2, T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2. |
| Source: | Pichia pastoris |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized from a 0.2�m filtered solution in 20mM sodium phosphate buffer pH 7.0 in absence of any carrier protein. |
| Solubility: | It is recommended to reconstitute the lyophilized Interleukin-2 in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Interleukin-2 should be stored at 4C between 2-7 days and for future use below -18C. Please prevent freeze-thaw cycles. |
| Purity: | Greater than 98% as determined by SDS-PAGE. |
| Amino Acid Sequence: | A P T S S S T K K T Q L Q L E H L L L D L Q M I L N G I N N Y K N P K L T R M L T F K F Y M P K K A T E L K H L Q C L E E E L K P L E E V L N L A Q S K N F H L R P R D L I S N I N V I V L E L K G S E T T F M C E Y A D E T A T I V E F L N R W I T F C Q S I I S T L T |
| Biological Activity: | The biological activity is determined by the dose dependent proliferation of mouse CTLL�2 cells. Optimal concentration for individual application should be determined by a dose response assay. ED50 range = 0.08�0.5ng/ml |
IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Interleukin-2 Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 14 kDa. The IL-2 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL2: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. Belongs to the IL-2 family. |
| UniProt Protein Details: | Protein type:Cytokine; Secreted; Secreted, signal peptide; Oncoprotein Chromosomal Location of Human Ortholog: 4q26-q27 Cellular Component: extracellular space; extracellular region Molecular Function:growth factor activity; interleukin-2 receptor binding; kinase activator activity; cytokine activity; carbohydrate binding; glycosphingolipid binding; kappa-type opioid receptor binding Biological Process: positive regulation of isotype switching to IgG isotypes; negative regulation of heart contraction; natural killer cell activation; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; elevation of cytosolic calcium ion concentration; negative regulation of protein amino acid phosphorylation; cell-cell signaling; protein kinase C activation; positive regulation of cell proliferation; positive regulation of B cell proliferation; cell adhesion; T cell differentiation; positive regulation of interleukin-17 production; regulation of T cell homeostatic proliferation; positive regulation of regulatory T cell differentiation; positive regulation of immunoglobulin secretion; positive regulation of cell growth; positive regulation of tissue remodeling; positive regulation of interferon-gamma production; positive regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; immune response; negative regulation of B cell apoptosis; positive regulation of inflammatory response; negative regulation of apoptosis |
| NCBI Summary: | The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P60568 |
| NCBI GenInfo Identifier: | 45593462 |
| NCBI Gene ID: | 3558 |
| NCBI Accession: | P60568.1 |
| UniProt Secondary Accession: | P60568,P01585, |
| UniProt Related Accession: | P60568 |
| Molecular Weight: | 153 |
| NCBI Full Name: | Interleukin-2 |
| NCBI Synonym Full Names: | interleukin 2 |
| NCBI Official Symbol: | IL2�� |
| NCBI Official Synonym Symbols: | IL-2; TCGF; lymphokine�� |
| NCBI Protein Information: | interleukin-2; aldesleukin; T cell growth factor; involved in regulation of T-cell clonal expansion |
| UniProt Protein Name: | Interleukin-2 |
| UniProt Synonym Protein Names: | T-cell growth factor; TCGF; INN: Aldesleukin |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL2�� |
| UniProt Entry Name: | IL2_HUMAN |
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