The Human IgM (Immunoglobulin M) ELISA Kit is specifically designed for the precise measurement of IgM levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an invaluable tool for various research purposes.Immunoglobulin M (IgM) is a primary antibody produced by the immune system in response to infections. Elevated levels of IgM can indicate an active infection, making it a vital biomarker for diagnosing and monitoring various infectious diseases.
The Human IgM ELISA Kit provides researchers with a reliable method for detecting and quantifying IgM levels, aiding in the understanding of immune responses and disease progression.Overall, the Human IgM ELISA Kit offers researchers a valuable tool for studying immune responses, infectious diseases, and other conditions where IgM plays a crucial role. Its high sensitivity and specificity make it a trusted choice for accurate and reproducible results in a wide range of applications.
Product Name:
Human IgM (Immunoglobulin M) ELISA Kit
SKU:
HUES02774
Target:
Human IgM (Immunoglobulin M)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IgM. You can calculate the concentration of Human IgM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-114
85-96
87-101
Average (%)
104
91
92
1:4
Range (%)
95-112
82-95
97-111
Average (%)
102
89
104
1:8
Range (%)
93-109
87-100
98-113
Average (%)
100
92
105
1:16
Range (%)
96-107
82-95
96-106
Average (%)
102
87
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
101
EDTA plasma (n=5)
96-108
102
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.6
26.7
81.3
9.9
28.3
81.9
Standard deviation
0.5
1.4
3.7
0.6
1.3
2.8
C V (%)
5.21
5.24
4.55
6.06
4.59
3.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IgM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IgM in samples. No significant cross-reactivity or interference between Human IgM and analogues was observed.
