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Human IgG4/Immunoglobulin heavy constant gamma 4 Polyclonal Antibody (CAB24655)

SKU:
CAB24655
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
$155 - $431
Frequently bought together:

Description

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Product Name:Human IgG4/Immunoglobulin heavy constant gamma 4 Polyclonal Antibody
SKU:CAB24655
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human IgG4/Immunoglobulin heavy constant gamma 4 (P01861).
Sequence:VHTF PAVL QSSG LYSL SSVV TVPS SSLG TKTY TCNV DHKP SNTK VDKR VESK YGPP CPSC PAPE FLGG PSVF LFPP KPKD TLMI SRTP EVTC VVVD VSQE D
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:IGHG4; Ig gamma-4 chain C region; Human IgG4/Immunoglobulin heavy constant gamma 4
Positive Sample:RPMI-8226,SH-SY5Y,Daudi,U-251 MG,K-562
Conjugate:Unconjugated
Cellular Localization:Secreted
Calculated MW:44kDa
Observed MW:36kDa

Immunoglobulin G (IgG), is one of the most abundant protein in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Purification Method:Affinity purification
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using Human IgG4/Immunoglobulin heavy constant gamma 4 Rabbit pAb (CAB24655) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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