The Human IgG4 (Immunoglobulin G4) ELISA Kit is a specialized assay designed for the precise and quantitative measurement of IgG4 levels in human biological samples. Immunoglobulin G4 is a subclass of antibodies that plays a crucial role in modulating immune responses and tolerance. This ELISA kit provides a reliable method for researchers to quantify IgG4 concentrations, offering insights into immune regulation, allergy responses, and autoimmune conditions in human samples. IgG4 antibodies are known for their anti-inflammatory properties and ability to counteract immune reactions. With its unique immunological functions, IgG4 contributes to maintaining immune homeostasis and preventing hypersensitivity reactions.
By accurately measuring IgG4 levels, researchers can evaluate immune tolerance, monitor therapeutic responses, and investigate autoimmune diseases with a focus on immune regulation. The Human IgG4 ELISA Kit features exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under rigorous quality control standards, this kit delivers reliable performance and user-friendly protocols, making it an excellent choice for research, clinical diagnostics, and immunological studies focusing on IgG4 antibody profiles in human samples.
Product Name:
Human IgG4 (Immunoglobulin G4) ELISA Kit
SKU:
AEES00652
Target:
Human IgG4 (Immunoglobulin G4)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.5h
Sensitivity:
0.38 μg/mL
Detection range:
0.63-40 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with IgG4. During the reaction, IgG4 in the sample or standard competes with a fixed amount of IgG4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to IgG4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IgG4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-111
88-100
97-111
Average (%)
102
94
105
1:4
Range (%)
98-110
84-99
99-114
Average (%)
104
90
107
1:8
Range (%)
90-103
87-100
101-115
Average (%)
97
92
107
1:16
Range (%)
93-109
85-97
98-109
Average (%)
100
92
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
99
EDTA plasma (n=5)
92-104
99
Cell culture media (n=5)
86-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.84
6.07
17.07
1.74
6.44
16.54
Standard deviation
0.13
0.32
0.68
0.1
0.33
0.73
C V (%)
6.85
5.34
3.97
5.78
5.11
4.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of IgG4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes IgG4 in samples. No significant cross-reactivity or interference between IgG4 and analogues was observed.
