The Human IgG4 (Immunoglobulin G4) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of IgG4 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a variety of research applications.IgG4 is a subtype of immunoglobulin G that plays a key role in immune responses and allergic reactions. Elevated levels of IgG4 have been associated with autoimmune diseases, allergic disorders, and certain types of cancer.
By measuring IgG4 levels, researchers can gain valuable insights into the underlying mechanisms of these conditions and potentially identify new therapeutic targets.With its high performance and ease of use, the Human IgG4 CLIA Kit from Assay Genie is a valuable tool for studying the role of IgG4 in health and disease. Order yours today and take your research to the next level.
Product Name:
Human IgG4 (Immunoglobulin G4) CLIA Kit
SKU:
HUES00975
Target:
Human IgG4 (Immunoglobulin G4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IgG4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgG4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgG4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IgG4. You can calculate the concentration of Human IgG4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-114
99-113
100-114
Average (%)
104
106
108
1:4
Range (%)
92-109
90-103
99-117
Average (%)
100
96
107
1:8
Range (%)
94-106
96-110
90-106
Average (%)
101
104
97
1:16
Range (%)
85-99
101-117
96-113
Average (%)
92
109
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
101
EDTA plasma (n=5)
85-97
90
Cell culture media (n=5)
83-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.78
15.36
47.58
4.91
16.8
43.1
Standard deviation
0.4
1.42
2.94
0.63
1.95
3.83
C V (%)
8.37
9.24
6.18
12.83
11.61
8.89
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IgG4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IgG4 in samples. No significant cross-reactivity or interference between Human IgG4 and analogues was observed.
