The Human IgG3 (Immunoglobulin G3) CLIA Kit from Assay Genie is specifically designed for the quantitative detection of IgG3 levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an excellent choice for various research applications.IgG3 is a subclass of immunoglobulins that plays a crucial role in immune responses, particularly in fighting against infections and supporting the body's defense mechanisms.
Monitoring IgG3 levels can provide valuable insights into immune system function and help in the diagnosis and treatment of various diseases and disorders.Whether you are studying autoimmune diseases, infectious diseases, or immune deficiencies, the Human IgG3 CLIA Kit offers a powerful tool for detecting and measuring IgG3 levels in human samples, allowing for in-depth analysis and investigation. Trust Assay Genie for reliable and high-quality research solutions.
Product Name:
Human IgG3 (Immunoglobulin G3) CLIA Kit
SKU:
HUES00974
Target:
Human IgG3 (Immunoglobulin G3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IgG3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgG3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgG3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IgG3. You can calculate the concentration of Human IgG3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
95-108
95-109
Average (%)
94
100
102
1:4
Range (%)
99-117
94-109
92-105
Average (%)
107
101
100
1:8
Range (%)
101-117
102-118
95-110
Average (%)
107
108
101
1:16
Range (%)
100-117
103-117
93-105
Average (%)
107
109
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
104
EDTA plasma (n=5)
93-105
98
Cell culture media (n=5)
100-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.63
243.99
961.69
97.63
229.6
1033.91
Standard deviation
11.55
20.35
105.31
12.51
24.2
90.36
C V (%)
12.21
8.34
10.95
12.81
10.54
8.74
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IgG3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IgG3 in samples. No significant cross-reactivity or interference between Human IgG3 and analogues was observed.
