The Human IgG2 (Immunoglobulin G2) ELISA Kit is a specialized assay designed for the quantitative measurement of IgG2 levels in human biological samples. IgG2 is a subclass of Immunoglobulin G antibodies that plays a crucial role in the adaptive immune response, particularly in responding to bacterial infections and toxins.
This ELISA kit enables the accurate detection and quantification of IgG2 antibodies, providing valuable insights into the immune status, response to pathogens, and antibody-mediated immunity in human samples. Immunoglobulin G2 antibodies are known for their ability to neutralize harmful pathogens, enhance immune responses, and maintain immune homeostasis. Monitoring IgG2 levels can help researchers assess immune function and identify immune system dysregulation in various conditions, including infectious diseases and autoimmune disorders. By quantifying IgG2 concentrations, this ELISA kit supports the investigation of immune responses, disease pathogenesis, and therapeutic outcomes in clinical and research settings. The Human IgG2 ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control standards, this kit delivers reliable performance and user-friendly protocols, making it a valuable tool for immunology research, clinical diagnostics, and antibody profiling studies in human samples.
Product Name:
Human IgG2 (Immunoglobulin G2) ELISA Kit
SKU:
AEES00650
Target:
Human IgG2 (Immunoglobulin G2)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.5h
Sensitivity:
0.61 μg/mL
Detection range:
1.56-100 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with IgG2. During the reaction, IgG2 in the sample or standard competes with a fixed amount of IgG2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to IgG2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IgG2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
101-116
85-100
Average (%)
95
106
92
1:4
Range (%)
86-101
97-113
83-98
Average (%)
93
104
89
1:8
Range (%)
91-103
99-114
82-96
Average (%)
97
105
88
1:16
Range (%)
87-97
97-111
82-93
Average (%)
92
102
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-110
100
EDTA plasma (n=5)
95-111
102
Cell culture media (n=5)
94-107
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.98
12.81
45.51
5.14
12.2
41.67
Standard deviation
0.29
0.61
2.41
0.27
0.73
1.57
C V (%)
5.9
4.8
5.29
5.23
6.0
3.77
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of IgG2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes IgG2 in samples. No significant cross-reactivity or interference between IgG2 and analogues was observed.
