The Human IgG1 (Immunoglobulin G1) ELISA Kit is a highly sensitive assay designed for the accurate quantification of IgG1 levels in human biological samples.
Immunoglobulin G1 is a major subclass of antibodies that plays a critical role in the humoral immune response, providing defense against pathogens, toxins, and foreign agents. This ELISA kit offers a reliable method for researchers to measure IgG1 concentrations, facilitating the investigation of immune function, antibody responses, and disease pathogenesis in human samples. IgG1 antibodies are known for their versatility in immune defense, including opsonization, neutralization, and antibody-dependent cell-mediated cytotoxicity. By detecting and quantifying IgG1 levels, researchers can gain insights into immune system activity, immunity to specific antigens, and antibody-mediated responses in various diseases and conditions. This ELISA kit provides a precise and reproducible means to assess IgG1 levels, supporting studies on immune status, vaccine response, and autoimmune disorders in research and clinical settings. The Human IgG1 ELISA Kit is characterized by exceptional sensitivity and specificity, ensuring accurate and trustworthy results. Manufactured with stringent quality control measures, this kit delivers robust performance and user-friendly protocols, offering researchers a valuable tool for immunology research, diagnostic investigations, and antibody profiling studies in human samples.
Product Name:
Human IgG1 (Immunoglobulin G1) ELISA Kit
SKU:
AEES00651
Target:
Human IgG1 (Immunoglobulin G1)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.5h
Sensitivity:
5.63 μg/mL
Detection range:
9.38-600 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with IgG1. During the reaction, IgG1 in the sample or standard competes with a fixed amount of IgG1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to IgG1. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IgG1 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
92-108
85-99
Average (%)
105
100
92
1:4
Range (%)
100-113
94-106
84-93
Average (%)
107
100
89
1:8
Range (%)
102-114
94-107
85-96
Average (%)
108
100
91
1:16
Range (%)
99-112
93-107
82-95
Average (%)
105
100
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
98
EDTA plasma (n=5)
96-110
101
Cell culture media (n=5)
87-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
30.33
93.09
220.66
30.79
85.03
226.42
Standard deviation
1.84
4.69
9.36
1.95
4.35
9.87
C V (%)
6.08
5.04
4.24
6.32
5.12
4.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of IgG1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes IgG1 in samples. No significant cross-reactivity or interference between IgG1 and analogues was observed.
