Human IGFBP-7 (Insulin Like Growth Factor Binding Protein 7) ELISA Kit
The Assay Genie Human IGFBP-7 (Insulin Like Growth Factor Binding Protein 7) ELISA Kit is expertly designed to quantitatively assess IGFBP-7 levels in a variety of biological samples. IGFBP-7, a crucial modulator of insulin-like growth factor (IGF) signaling, plays a significant role in regulating cellular growth, development, and differentiation processes. By accurately measuring IGFBP-7 levels, researchers can gain valuable insights into the intricate mechanisms underlying growth factor interactions and their impact on various physiological and pathological conditions.
Our IGFBP-7 ELISA Kit ensures exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for your research endeavors. With stringent quality control measures in place during manufacturing, this kit delivers robust performance and user-friendly protocols, making it an ideal choice for conducting precise and reliable quantification studies in the realm of IGFBP-7-related research. Trust Assay Genie's IGFBP-7 ELISA Kit for dependable and high-quality analysis, empowering your scientific investigations.
Product Name:
Human IGFBP-7 (Insulin Like Growth Factor Binding Protein 7) ELISA Kit
SKU:
AEES00176
Target:
Human IGFBP-7 (Insulin Like Growth Factor Binding Protein 7)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP-7. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGFBP-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGFBP-7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IGFBP-7. You can calculate the concentration of Human IGFBP-7 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
91-101
90-102
Average (%)
97
96
97
1:4
Range (%)
87-100
88-100
87-99
Average (%)
95
95
93
1:8
Range (%)
91-103
89-102
87-99
Average (%)
98
94
93
1:16
Range (%)
88-102
85-96
88-103
Average (%)
94
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
91
EDTA plasma (n=5)
96-107
101
Cell culture media (n=5)
90-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.33
264.92
952.33
91.69
263.98
1011.09
Standard deviation
6.17
13.27
45.14
6.32
10.59
36.2
C V (%)
6.76
5.01
4.74
6.89
4.01
3.58
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IGFBP-7 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGFBP-7 in samples. No significant cross-reactivity or interference between Human IGFBP-7 and analogues was observed.
