Human IGFALS (Insulin Like Growth Factor Binding Protein, Acid Labile Subunit) CLIA Kit
The Human IGFALS (Insulin-Like Growth Factor Binding Protein Acid Labile Subunit) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of IGFALS levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it the ideal tool for a wide range of research applications.IGFALS is a critical protein that binds to insulin-like growth factors, regulating their bioavailability and activity. Dysregulation of IGFALS has been implicated in various diseases, including metabolic disorders and growth-related conditions.
Studying IGFALS levels can provide valuable insights into these diseases and potentially aid in the development of new therapeutic interventions.This CLIA kit offers researchers a convenient and efficient way to quantify IGFALS levels in biological samples, enabling them to study the role of this protein in health and disease. Trust in the accuracy and precision of this kit to advance your research in the field of growth factor biology.
Product Name:
Human IGFALS (Insulin Like Growth Factor Binding Protein, Acid Labile Subunit) CLIA Kit
SKU:
HUES00318
Target:
Human IGFALS (Insulin Like Growth Factor Binding Protein, Acid Labile Subunit)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IGFALS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGFALS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGFALS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IGFALS. You can calculate the concentration of Human IGFALS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
92-105
91-104
Average (%)
95
99
98
1:4
Range (%)
102-119
98-114
86-98
Average (%)
109
104
92
1:8
Range (%)
87-98
99-116
90-105
Average (%)
93
107
97
1:16
Range (%)
85-96
101-113
88-103
Average (%)
90
106
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
95
EDTA plasma (n=5)
100-115
108
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.0
3.15
9.19
0.9
3.34
8.6
Standard deviation
0.13
0.28
1.02
0.08
0.36
0.93
C V (%)
13.0
8.89
11.1
8.89
10.78
10.81
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IGFALS concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGFALS in samples. No significant cross-reactivity or interference between Human IGFALS and analogues was observed.
