Human IGF-1 (Insulin-Like Growth Factor 1) ELISA Kit
The Human IGF-1 (Insulin-like Growth Factor 1) ELISA Kit is specifically designed for the precise measurement of IGF-1 levels in human serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a diverse range of research applications.IGF-1, a key growth factor, plays a critical role in promoting cell growth, differentiation, and metabolism. It is closely associated with various physiological processes, including tissue repair, muscle growth, and bone development.
Dysregulation of IGF-1 levels has been linked to a variety of disorders, such as diabetes, cancer, and growth deficiencies, making it a valuable biomarker for understanding disease mechanisms and potential therapeutic interventions.Overall, the Human IGF-1 ELISA Kit provides researchers with a powerful tool for investigating the role of IGF-1 in health and disease, offering valuable insights into cellular processes and opening new avenues for innovative research and treatment strategies.
Product Name:
Human IGF-1 (Insulin-Like Growth Factor 1) ELISA Kit
SKU:
HUES01372
Target:
Human IGF-1 (Insulin-Like Growth Factor 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGF-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGF-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IGF-1. You can calculate the concentration of Human IGF-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
97-109
99-113
Average (%)
91
103
106
1:4
Range (%)
95-110
88-98
96-112
Average (%)
101
93
104
1:8
Range (%)
99-112
81-91
96-111
Average (%)
105
86
103
1:16
Range (%)
94-106
82-96
93-105
Average (%)
100
88
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
100
EDTA plasma (n=5)
92-108
99
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.3
11.8
48.6
5.6
10.9
47.7
Standard deviation
0.3
0.5
1.9
0.3
0.5
1.8
C V (%)
5.66
4.24
3.91
5.36
4.59
3.77
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IGF-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGF-1 in samples. No significant cross-reactivity or interference between Human IGF-1 and analogues was observed.
