The Human IgA2 (Immunoglobulin A2) ELISA Kit is meticulously developed for the precise and quantitative assessment of Immunoglobulin A2 levels in diverse biological samples. Immunoglobulin A2, a crucial component of the body's immune system, plays a fundamental role in providing immunity at mucosal surfaces, protecting against infections and pathogens. This ELISA kit facilitates the accurate detection of IgA2, enabling detailed insights into immune responses, mucosal immunity, and various immune-related disorders.
With exceptional sensitivity and specificity, the kit ensures accurate and reproducible results. Manufactured under strict quality control standards, this kit guarantees robust performance and user-friendly operation, making it an ideal choice for both research and clinical applications. Rely on the Human IgA2 ELISA Kit for precise and reliable quantification of this vital immunoglobulin in your research endeavors.
Product Name:
Human IgA2 (Immunoglobulin A2) ELISA Kit
SKU:
AEES00110
Target:
Human IgA2 (Immunoglobulin A2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgA2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IgA2. You can calculate the concentration of Human IgA2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-105
97-107
92-99
Average (%)
98
104
95
1:4
Range (%)
89-98
100-107
90-104
Average (%)
92
103
97
1:8
Range (%)
92-105
88-100
98-109
Average (%)
100
92
104
1:16
Range (%)
100-113
97-106
88-99
Average (%)
105
102
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
94-108
100
Cell culture media (n=5)
88-95
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.81
10.86
43.21
4.49
10.95
44.4
Standard deviation
0.24
0.52
1.84
0.24
0.54
2.22
C V (%)
4.99
4.79
4.26
5.35
4.93
5.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IgA2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IgA2 in samples. No significant cross-reactivity or interference between Human IgA2 and analogues was observed.
