The Human IFNα (Interferon Alpha) CLIA Kit is a cutting-edge assay meticulously crafted to precisely quantify Interferon Alpha levels in human samples. IFNα, an essential type I interferon, plays a pivotal role in orchestrating the body’s immune response against viral infections, tumors, and other immune-related pathways.
Its potent antiviral and immunomodulatory properties are crucial for host defense mechanisms, immune surveillance, and immunity regulation, highlighting its significance in immune homeostasis and disease pathogenesis. With a focus on immune system functionality, the IFNα CLIA Kit offers researchers and clinicians a state-of-the-art tool for quantifying IFNα levels accurately. By enabling the quantification of Interferon Alpha, this kit facilitates in-depth investigations into viral infections, autoimmune diseases, and cancer immunotherapy efficacy. Characterized by exceptional sensitivity and specificity, the Human IFNα CLIA Kit delivers dependable and reproducible results, ensuring reliable performance and precise quantitation of IFNα across diverse biological samples. Manufactured under rigorous quality assurance processes, this kit guarantees robust and user-friendly performance, making it an excellent selection for advanced research, clinical diagnostics, and immunological studies.
Product Name:
Human IFNα (Interferon Alpha) CLIA Kit
SKU:
AEES00729
Target:
Human IFNα (Interferon Alpha)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IFNα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFNα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFNα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IFNα. You can calculate the concentration of Human IFNα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
100-113
94-105
Average (%)
90
105
98
1:4
Range (%)
92-106
91-102
91-103
Average (%)
98
99
96
1:8
Range (%)
98-111
94-105
84-100
Average (%)
105
101
92
1:16
Range (%)
93-102
91-103
86-100
Average (%)
97
95
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
94
EDTA plasma (n=5)
100-108
103
Cell culture media (n=5)
98-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.7
50.47
218.78
21.97
61.62
235.23
Standard deviation
1.32
2.48
11.86
1.31
2.94
11.27
C V (%)
5.34
4.91
5.42
5.96
4.77
4.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IFNα concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IFNα in samples. No significant cross-reactivity or interference between Human IFNα and analogues was observed.
