Human IFABP (Intestinal Fatty Acid Binding Protein) ELISA Kit
The Human IFABP (Intestinal Fatty Acid Binding Protein) ELISA Kit is a highly sensitive and specific assay designed for the precise detection of IFABP levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it suitable for various research applications.IFABP is an important protein involved in the transport of fatty acids in the intestines, playing a critical role in lipid metabolism and absorption.
Abnormal levels of IFABP have been implicated in various gastrointestinal disorders, making it a valuable biomarker for studying these conditions and developing potential interventions.Overall, the Human IFABP ELISA kit is a valuable tool for researchers looking to investigate the role of IFABP in health and disease, providing accurate and quantitative measurements for a deeper understanding of intestinal fatty acid binding protein function.
Product Name:
Human IFABP (Intestinal Fatty Acid Binding Protein) ELISA Kit
SKU:
HUES01431
Target:
Human IFABP (Intestinal Fatty Acid Binding Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFABP/FABP2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFABP/FABP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFABP/FABP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IFABP/FABP2. You can calculate the concentration of Human IFABP/FABP2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-99
86-100
94-109
Average (%)
94
93
99
1:4
Range (%)
92-104
81-91
88-101
Average (%)
97
86
93
1:8
Range (%)
87-101
86-100
84-97
Average (%)
94
93
90
1:16
Range (%)
91-106
82-96
85-96
Average (%)
97
89
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
98
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
89-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.11
3.94
0.5
1.11
4.25
Standard deviation
0.03
0.07
0.13
0.03
0.06
0.16
C V (%)
5.45
6.31
3.3
6.0
5.41
3.76
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IFABP/FABP2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IFABP/FABP2 in samples. No significant cross-reactivity or interference between Human IFABP/FABP2 and analogues was observed.
