The Human Complement iC3b (iC3b) ELISA Kit enables the precise quantitative measurement of iC3b levels in diverse human biological samples.
iC3b is a crucial component of the complement system involved in immune responses, opsonization, and clearance of immune complexes. As a fragment of complement component 3 (C3), iC3b plays a fundamental role in orchestrating immune defense mechanisms by promoting phagocytosis and modulating inflammatory processes. Our iC3b ELISA Kit from Assay Genie offers outstanding sensitivity and specificity, ensuring accurate and reproducible results essential for investigating the roles of iC3b in various physiological and pathological conditions. With stringent quality controls in place, this kit guarantees robust performance and user-friendly protocols, making it an ideal choice for research applications. Trust Assay Genie's iC3b ELISA Kit for the precise quantification of this pivotal biomarker in your scientific studies.
Product Name:
Human iC3b (Complement iC3b) ELISA Kit
SKU:
AEES00143
Target:
Human iC3b (Complement iC3b)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human iC3b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human iC3b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human iC3b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human iC3b. You can calculate the concentration of Human iC3b in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-97
89-104
88-100
Average (%)
92
96
93
1:4
Range (%)
98-106
91-100
91-101
Average (%)
101
96
94
1:8
Range (%)
97-108
85-100
89-101
Average (%)
102
93
93
1:16
Range (%)
101-111
92-105
95-106
Average (%)
105
98
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
91-99
94
Cell culture media (n=5)
95-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
8.04
21.36
94.54
9.91
22.31
87.35
Standard deviation
0.48
0.99
4.3
0.54
1.07
3.56
C V (%)
5.97
4.63
4.55
5.45
4.8
4.08
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human iC3b concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human iC3b in samples. No significant cross-reactivity or interference between Human iC3b and analogues was observed.
