The Human I-PTH (intact Parathormone) ELISA Kit is meticulously crafted for the precise and quantitative detection of intact Parathormone in a variety of biological samples. Intact Parathormone plays a fundamental role in calcium and phosphorus metabolism by regulating calcium levels in the blood through its actions on the bones, kidneys, and intestines. This ELISA kit allows for accurate measurement of intact Parathormone levels, providing valuable insights into bone health, vitamin D metabolism, and calcium homeostasis.
With exceptional sensitivity and specificity, the kit ensures reliable and reproducible results. Manufactured under stringent quality control standards, this kit delivers robust performance and user-friendly protocols, making it an excellent choice for both research and clinical applications. Trust the Human I-PTH ELISA Kit for accurate and dependable quantification of this essential biomarker in your studies.
Product Name:
Human I-PTH (intact Parathormone) ELISA Kit
SKU:
AEES00112
Target:
Human I-PTH (intact Parathormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human I-PTH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human I-PTH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human I-PTH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human I-PTH. You can calculate the concentration of Human I-PTH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-102
85-96
95-106
Average (%)
99
90
101
1:4
Range (%)
95-106
84-97
86-97
Average (%)
99
90
93
1:8
Range (%)
99-113
100-110
93-106
Average (%)
105
103
101
1:16
Range (%)
92-106
98-106
93-107
Average (%)
98
101
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
96
EDTA plasma (n=5)
96-106
102
Cell culture media (n=5)
98-107
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.06
20.89
97.51
9.32
22.38
97.93
Standard deviation
0.52
1.04
4.43
0.54
1.07
4.73
C V (%)
5.74
4.98
4.54
5.79
4.78
4.83
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human I-PTH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human I-PTH in samples. No significant cross-reactivity or interference between Human I-PTH and analogues was observed.
