Human HYOU1 (Hypoxia Up Regulated 1) CLIA Kit (HUES00308)
- SKU:
- HUES00308
- Product Type:
- ELISA Kit
- ELISA Type:
- CLIA Kit
- Size:
- 96 Assays
- Sensitivity:
- 18.75pg/mL
- Range:
- 31.25-2000pg/mL
- ELISA Type:
- Sandwich
- Synonyms:
- Grp170, HSP12A, ORP150
- Reactivity:
- Human
- Sample Type:
- Serum, plasma and other biological fluids
- Research Area:
- Metabolism
Description
Product Name: | Human HYOU1 (Hypoxia Up Regulated 1) CLIA Kit |
SKU: | HUES00308 |
Target: | Human HYOU1 (Hypoxia Up Regulated 1) |
Size: | 96T |
Assay type: | Sandwich |
Assay time: | 4.5h |
Sensitivity: | 18.75 pg/mL |
Detection range: | 31.25-2000 pg/mL |
Kit component: |
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This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HYOU1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HYOU1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HYOU1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HYOU1. You can calculate the concentration of Human HYOU1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity: |
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Recovery: |
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Precision: |
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Sample type &Sample volume: | Tissue homogenates; 100μL |
Reproducibility: | Both intra-CV and inter-CV are < 15%. |
Application: | This CLIA kit applies to the in vitro quantitative determination of Human HYOU1 concentrations in Tissue homogenates. |
Specificity: | This kit recognizes Human HYOU1 in samples. No significant cross-reactivity or interference between Human HYOU1 and analogues was observed. |