The Human Hyaluronidase (HAase) ELISA Kit is specifically developed to accurately measure the levels of hyaluronidase in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and consistent results, making it an invaluable tool for various research purposes.Hyaluronidase is an enzyme that plays a critical role in breaking down hyaluronic acid, a key component of extracellular matrix, which is essential for cell migration and tissue remodeling. Dysregulation of hyaluronidase activity has been linked to various pathological conditions such as cancer, inflammation, and tissue damage, highlighting the importance of monitoring its levels in biological samples.
By using the Human Hyaluronidase (HAase) ELISA Kit, researchers can gain valuable insights into the role of hyaluronidase in disease progression and potentially identify new therapeutic targets for intervention. With its reliable performance and user-friendly design, this kit is a valuable asset for any laboratory conducting studies on hyaluronidase biology and related disorders.
Product Name:
Human Hyaluronidase (HAase) ELISA Kit
Product Code:
HUDL01295
Size:
96 Assays
Target:
HAase
Synonyms:
Hyaluronic Lase, Hyaluroniclase
Detection Method:
Sandwich
Range:
3.125-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
90-102
96
EDTA plasma (n=5)
90-101
95
Heparin plasma (n=5)
91-98
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
