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Human Hyaluronan Binding Protein 1 (HABP1) ELISA Kit

SKU:
HUDL01296
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q07021
Sensitivity:
0.072ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
C1QBP,GC1QBP,SF2p32,GC1Q-R,GC1qR,P32,Complement Component 1 Q Subcomponent Binding Protein,Splicing Factor SF2-Associated Protein,ASF/SF2-associated p32
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Hyaluronan Binding Protein 1 (HABP1) ELISA Kit

The Human Hyaluronan Binding Protein 1 (HABP1) ELISA Kit is a powerful tool for detecting levels of HABP1 in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results, making it an invaluable tool for researchers in various fields.Hyaluronan Binding Protein 1 (HABP1) is a key protein involved in binding to hyaluronic acid, an important component of the extracellular matrix.

HABP1 plays a critical role in cell adhesion, migration, and proliferation, making it a valuable biomarker for studying cellular processes and disease mechanisms.Whether you are studying cell signaling pathways, cancer biology, or tissue regeneration, the Human HABP1 ELISA Kit offers a reliable solution for measuring HABP1 levels in human samples. Trust in this kit for consistent and precise results in your research endeavors.

Product Name:Human Hyaluronan Binding Protein 1 (HABP1) ELISA Kit
Product Code:HUDL01296
Size:96 Assays
Target:HABP1
Synonyms:C1QBP, GC1QBP, SF2p32, GC1Q-R, GC1qR, P32, Complement Component 1 Q Subcomponent Binding Protein, Splicing Factor SF2-Associated Protein, ASF/SF2-associated p32
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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