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Human HS3ST1 Recombinant Protein (RPPB1831)

SKU:
RPPB1831
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O14792
Research Area:
Enzymes
$371
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Description

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Product Name:Human HS3ST1 Recombinant Protein
Product Code:RPPB1831
Size:10µg
Species:Human
Target:HS3ST1
Synonyms:Heparan sulfate glucosamine 3-O-sulfotransferase 1, Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1, 3-OST-1, Heparan sulfate 3-O-sulfotransferase 1, h3-OST-1, HS3ST1, 3OST, 3OST1, HS3S1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:HS3ST1 protein solution (0. 25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol and 2mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSRPAELGQ QELLRKAGTL QDDVRDGVAP NGSAQQLPQT IIIGVRKGGT RALLEMLSLH PDVAAAENEV HFFDWEEHYS HGLGWYLSQM PFSWPHQLTV EKTPAYFTSP KVPERVYSMN PSIRLLLILR DPSERVLSDY TQVFYNHMQK HKPYPSIEEF LVRDGRLNVD YKALNRSLYH VHMQNWLRFF PLRHIHIVDG DRLIRDPFPE IQKVERFLKL SPQINASNFY FNKTKGFYCL RDSGRDRCLH ESKGRAHPQV DPKLLNKLHE YFHEPNKKFF ELVGRTFDWH

Heparan Sulfate 3-O-Sulfotransferase 1 (HS3ST1), is sulfotransferase which uses 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. HS3ST1 catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a vital part in the biosynthesis of anticoagulant heparan sulfate since it concludes the structure of the antithrombin pentasaccharide binding site.

HS3ST1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 310 amino acids (21-307 a.a) and having a molecular mass of 36.2kDa.HS3ST1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:HS3ST1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site. Belongs to the sulfotransferase 1 family.Protein type: EC 2.8.2.23; Transferase; Glycan Metabolism - heparan sulfate biosynthesisChromosomal Location of Human Ortholog: 4p16Cellular Component: Golgi lumen; integral to membraneMolecular Function: sulfotransferase activity; heparin-glucosamine 3-O-sulfotransferase activityBiological Process: glycosaminoglycan biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis
UniProt Protein Details:
NCBI Summary:Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It possesses both heparan sulfate glucosaminyl 3-O-sulfotransferase activity, anticoagulant heparan sulfate conversion activity, and is a rate limiting enzyme for synthesis of anticoagulant heparan. This enzyme is an intraluminal Golgi resident protein. [provided by RefSeq, Jul 2008]
UniProt Code:O14792
NCBI GenInfo Identifier:4826764
NCBI Gene ID:9957
NCBI Accession:NP_005105.1
UniProt Secondary Accession:O14792,Q6PEY8, B3KUA6
UniProt Related Accession:O14792
Molecular Weight:35,773 Da
NCBI Full Name:heparan sulfate glucosamine 3-O-sulfotransferase 1
NCBI Synonym Full Names:heparan sulfate (glucosamine) 3-O-sulfotransferase 1
NCBI Official Symbol:HS3ST1��
NCBI Official Synonym Symbols:3OST; 3OST1��
NCBI Protein Information:heparan sulfate glucosamine 3-O-sulfotransferase 1; 3-OST-1; h3-OST-1; heparan sulfate 3-O-sulfotransferase 1; heparin-glucosamine 3-O-sulfotransferase; heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1
UniProt Protein Name:Heparan sulfate glucosamine 3-O-sulfotransferase 1
UniProt Synonym Protein Names:Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1; 3-OST-1; Heparan sulfate 3-O-sulfotransferase 1; h3-OST-1
Protein Family:
UniProt Gene Name:HS3ST1��
UniProt Entry Name:HS3S1_HUMAN

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