The Human HS (Heparan Sulfate) ELISA Kit is a highly reliable and sensitive tool for the precise detection of heparan sulfate levels in human samples including serum, plasma, and cell culture supernatants. With its high specificity and accuracy, this kit provides consistent and reproducible results, making it ideal for a variety of research purposes.Heparan sulfate is a critical component of the extracellular matrix, playing a key role in various physiological and pathological processes including cell signaling, growth factor binding, and development.
Abnormalities in heparan sulfate levels have been linked to diseases such as cancer, diabetes, and inflammatory disorders, highlighting its importance as a biomarker for disease diagnosis and therapeutic development.With the Human HS ELISA Kit, researchers can confidently study the role of heparan sulfate in disease progression and explore potential treatment strategies with confidence and precision.
Product Name:
Human HS (heparan sulfate) ELISA Kit
SKU:
HUES03235
Target:
Human HS (heparan sulfate)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HS. You can calculate the concentration of Human HS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
95-108
96-110
Average (%)
103
102
104
1:4
Range (%)
92-107
84-96
88-102
Average (%)
98
90
94
1:8
Range (%)
89-105
83-96
86-97
Average (%)
96
88
92
1:16
Range (%)
92-105
86-99
84-99
Average (%)
98
92
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
96
EDTA plasma (n=5)
96-108
102
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.95
2.16
7.34
0.99
1.95
7.15
Standard deviation
0.06
0.1
0.34
0.05
0.11
0.35
C V (%)
6.32
4.63
4.63
5.05
5.64
4.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HS in samples. No significant cross-reactivity or interference between Human HS and analogues was observed.
