The Human HPA (Heparanase) ELISA Kit is a specialized assay designed for the precise measurement of heparanase levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Heparanase is a critical enzyme involved in heparan sulfate degradation, which plays a key role in various physiological and pathological processes including tumor metastasis, inflammation, and angiogenesis.
By accurately measuring heparanase levels, researchers can gain valuable insights into the role of this enzyme in disease progression and potentially identify new therapeutic targets.Overall, the Human HPA (Heparanase) ELISA Kit is an essential tool for studying the role of heparanase in health and disease, offering reliable and precise measurements to advance scientific research and drug development efforts.
Product Name:
Human HPA (Heparanase) ELISA Kit
SKU:
HUES03236
Target:
Human HPA (Heparanase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HPA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HPA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HPA. You can calculate the concentration of Human HPA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
100-116
94-108
Average (%)
96
106
101
1:4
Range (%)
93-105
84-98
85-97
Average (%)
98
91
91
1:8
Range (%)
85-100
80-92
85-99
Average (%)
92
86
90
1:16
Range (%)
85-99
84-96
88-98
Average (%)
92
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-104
98
EDTA plasma (n=5)
87-102
94
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.09
4.17
0.53
1.15
4.57
Standard deviation
0.04
0.05
0.13
0.04
0.05
0.19
C V (%)
7.41
4.59
3.12
7.55
4.35
4.16
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HPA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HPA in samples. No significant cross-reactivity or interference between Human HPA and analogues was observed.
