The Human HNP1-3 (Neutrophil Peptide 1-3) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of HNP1-3 levels in human biological samples. This kit is suitable for the measurement of HNP1-3 in serum, plasma, and cell culture supernatants, providing researchers with reliable and reproducible data for a variety of research applications.HNP1-3 are antimicrobial peptides produced by neutrophils, playing a key role in the immune response to infection and inflammation. Elevated levels of HNP1-3 have been associated with various inflammatory diseases, making them valuable biomarkers for monitoring disease progression and evaluating treatment efficacy.
By using the Human HNP1-3 ELISA Kit, researchers can gain valuable insights into the role of HNP1-3 in disease pathogenesis and potentially identify new therapeutic targets for the treatment of inflammatory disorders. With its high sensitivity and specificity, this kit is an essential tool for advancing research in the field of immunology and inflammation.
Product Name:
Human HNP1-3 (Neutrophil Peptide 1-3) ELISA Kit
SKU:
HUES03176
Target:
Human HNP1-3 (Neutrophil Peptide 1-3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HNP1-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HNP1-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HNP1-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HNP1-3. You can calculate the concentration of Human HNP1-3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
100-112
100-115
Average (%)
100
106
105
1:4
Range (%)
92-105
88-101
85-97
Average (%)
99
93
92
1:8
Range (%)
93-107
78-91
86-96
Average (%)
100
85
91
1:16
Range (%)
89-101
80-95
83-97
Average (%)
94
87
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-101
92
EDTA plasma (n=5)
91-105
97
Cell culture media (n=5)
86-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.91
4.86
16.61
2.06
5.3
15.84
Standard deviation
0.12
0.23
0.75
0.13
0.3
0.64
C V (%)
6.28
4.73
4.52
6.31
5.66
4.04
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HNP1-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HNP1-3 in samples. No significant cross-reactivity or interference between Human HNP1-3 and analogues was observed.
