The Human HNP1-3 (Neutrophil Peptide 1-3) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of HNP1-3 levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.HNP1-3, also known as human defensins, are small cationic antimicrobial peptides produced by neutrophils in response to infection or inflammation. These peptides play a critical role in the innate immune response by targeting and killing pathogens.
Elevated levels of HNP1-3 have been associated with various infectious and inflammatory conditions, making it a valuable biomarker for studying these diseases and monitoring treatment efficacy.The Human HNP1-3 CLIA Kit is a valuable tool for researchers studying the role of defensins in the immune response, infectious diseases, and inflammatory disorders. Its high sensitivity and specificity ensure accurate quantification of HNP1-3 levels, providing valuable insights into the pathogenesis of various diseases and potential therapeutic targets.
Product Name:
Human HNP1-3 (Neutrophil Peptide 1-3) CLIA Kit
SKU:
HUES01195
Target:
Human HNP1-3 (Neutrophil Peptide 1-3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HNP1-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HNP1-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HNP1-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HNP1-3. You can calculate the concentration of Human HNP1-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-97
97-109
86-99
Average (%)
92
103
92
1:4
Range (%)
90-102
99-113
87-99
Average (%)
96
107
93
1:8
Range (%)
102-114
84-98
91-105
Average (%)
108
90
98
1:16
Range (%)
86-100
89-105
101-115
Average (%)
92
96
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
102
EDTA plasma (n=5)
94-107
100
Cell culture media (n=5)
90-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
408.16
1123.21
3551.02
421.76
1064.2
3378.42
Standard deviation
38.49
127.6
237.56
39.43
84.71
216.89
C V (%)
9.43
11.36
6.69
9.35
7.96
6.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HNP1-3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HNP1-3 in samples. No significant cross-reactivity or interference between Human HNP1-3 and analogues was observed.
