Human HNMT Recombinant Protein (RPPB1818)
- SKU:
- RPPB1818
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P50135
- Research Area:
- Enzymes
Description
| Product Name: | Human HNMT Recombinant Protein |
| Product Code: | RPPB1818 |
| Size: | 20µg |
| Species: | Human |
| Target: | HNMT |
| Synonyms: | HMT, HNMT-S1, HNMT-S2, HNMT, Histamine N-methyltransferase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear colorless solution. |
| Formulation: | The HNMT solution contains 20mM Tris-HCl pH-8 and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASS MRSLFSDHGK YVESFRRFLN HSTEHQCMQE FMDKKLPGII GRIGDTKSEI KILSIGGGAG EIDLQILSKV QAQYPGVCIN NEVVEPSAEQ IAKYKELVAK TSNLENVKFA WHKETSSEYQ SRMLEKKELQ KWDFIHMIQM LYYVKDIPAT LKFFHSLLGT NAKMLIIVVS GSSGWDKLWK KYGSRFPQDD LCQYITSDDL TQMLDNLGLK YECYDLLSTM DISDCFIDGD ENGDLLWDFL TETCNFNATA PPDLRAELGK DLQEPEFSAK KEGKVLFNNT LSFIVIEA |
HNMT is located in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammal�s brain,N(tau)-methylation controls the neurotransmitter activity of histamine since diamine oxidase is not located in the central nervous system. A well known genetic polymorphism influences the activity levels of HNMT gene product in red blood cells. HNMT inactivates histamine by n-methylation. HNMT is involved in degrading histamine and in regulating the airway response to histamine. Histamine is involved in regulation and modulation of immune response through the stimulation of four distinct subtypes of receptors, H1, H2, H3, and H4, that present on the target cells. Histamine is inactivated by the histamine-metabolizing enzyme HNMT in bronchus, kidney, and the central nervous system.
HNMT Human Recombinant fused to 36 amino acid His Tag at N-terminal produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 328 amino acids (1-292) and having a molecular mass of 37 kDa. The HNMT is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | HNMT: Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine. Belongs to the methyltransferase superfamily. HNMT family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - histidine; EC 2.1.1.8; Methyltransferase Chromosomal Location of Human Ortholog: 2q22.1 Cellular Component: cytoplasm; cytosol; neuron projection; nucleoplasm Molecular Function:histamine N-methyltransferase activity Biological Process: brain development; histidine catabolic process; methylation; respiratory gaseous exchange Disease: Asthma, Susceptibility To |
| NCBI Summary: | In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P50135 |
| NCBI GenInfo Identifier: | 1708272 |
| NCBI Gene ID: | 3176 |
| NCBI Accession: | P50135.1 |
| UniProt Secondary Accession: | P50135,Q546Z6, Q7Z7I2, Q8IU56, Q8WW98, Q9BRW6, B2R9J3 |
| UniProt Related Accession: | P50135 |
| Molecular Weight: | 6,046 Da |
| NCBI Full Name: | Histamine N-methyltransferase |
| NCBI Synonym Full Names: | histamine N-methyltransferase |
| NCBI Official Symbol: | HNMT�� |
| NCBI Official Synonym Symbols: | HMT; MRT51; HNMT-S1; HNMT-S2�� |
| NCBI Protein Information: | histamine N-methyltransferase |
| UniProt Protein Name: | Histamine N-methyltransferase |
| Protein Family: | Histamine N-methyltransferase |
| UniProt Gene Name: | HNMT�� |
| UniProt Entry Name: | HNMT_HUMAN |
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