Human HLA-DOB Recombinant Protein (RPPB3688)
- SKU:
- RPPB3688
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P13765
Description
| Product Name: | Human HLA-DOB Recombinant Protein |
| Product Code: | RPPB3688 |
| Size: | 20µg |
| Species: | Human |
| Target: | HLA-DOB |
| Synonyms: | HLA class II histocompatibility antigen, DO beta chain, MHC class II antigen DOB, HLA-DOB, Major histocompatibility complex, class II, DO beta, DOB. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The HLA-DOB solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSGTDSPED FVIQAKADCY FTNGTEKVQF VVRFIFNLEE YVRFDSDVGM FVALTKLGQP DAEQWNSRLD LLERSRQAVD GVCRHNYRLG APFTVGRKVQ PEVTVYPERT PLLHQHNLLH CSVTGFYPGD IKIKWFLNGQ EERAGVMSTG PIRNGDWTFQ TVVMLEMTPE LGHVYTCLVD HSSLLSPVSV EWRAQSEYSW RK |
Major Histocompatibility Complex Class II DO Beta (HLA-DOB) is a part of the HLA class II beta chain paralogues. This class II beta chain is a heterodimer which is located in intracellular vesicles and consists of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. HLA-DOB which interacts with the HLA-DM molecule in B-cells is a significant modulator in the HLA class II restricted antigen presentation pathway. Class II molecules are expressed in antigen presenting cells such as B lymphocytes, dendritic cells and macrophages.
HLA-DOB Human Recombinant produced in E. coli is a single polypeptide chain containing 222 amino acids (27-224) and having a molecular mass of 25.2 kDa.HLA-DOB is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | HLA-DOB: Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM. Belongs to the MHC class II family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.32 Cellular Component: lysosomal membrane; lysosome; MHC class II protein complex Molecular Function:MHC class II receptor activity Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; negative regulation of antigen processing and presentation of peptide antigen via MHC class II |
| NCBI Summary: | HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P13765 |
| NCBI GenInfo Identifier: | 122261 |
| NCBI Gene ID: | 3112 |
| NCBI Accession: | P13765.1 |
| UniProt Secondary Accession: | P13765,Q29746, Q29825, Q6FHC2, B0V0Y0, |
| UniProt Related Accession: | P13765 |
| Molecular Weight: | 31kDa |
| NCBI Full Name: | HLA class II histocompatibility antigen, DO beta chain |
| NCBI Synonym Full Names: | major histocompatibility complex, class II, DO beta |
| NCBI Official Symbol: | HLA-DOB�� |
| NCBI Official Synonym Symbols: | DOB; HLA_DOB�� |
| NCBI Protein Information: | HLA class II histocompatibility antigen, DO beta chain |
| UniProt Protein Name: | HLA class II histocompatibility antigen, DO beta chain |
| UniProt Synonym Protein Names: | MHC class II antigen DOB |
| Protein Family: | HLA class II histocompatibility antigen |
| UniProt Gene Name: | HLA-DOB�� |
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