Human HLA-B27 (Human Leukocyte Antigen B27) CLIA Kit
The Human HLA-B27 (Human Leukocyte Antigen-B27) CLIA Kit is a cutting-edge diagnostic tool designed for accurate detection of HLA-B27 levels in human serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, providing researchers and healthcare professionals with reliable and consistent results for a variety of applications.HLA-B27 is a vital component of the immune system, playing a crucial role in autoimmune diseases such as ankylosing spondylitis and reactive arthritis.
Detecting HLA-B27 levels can aid in early diagnosis, monitoring disease progression, and assessing treatment effectiveness. This CLIA kit is essential for studying these conditions and developing targeted therapies to improve patient outcomes.
Product Name:
Human HLA-B27 (Human Leukocyte Antigen B27) CLIA Kit
SKU:
HUES00137
Target:
Human HLA-B27 (Human Leukocyte Antigen B27)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HLA-B27. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HLA-B27 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HLA-B27, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HLA-B27. You can calculate the concentration of Human HLA-B27 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
96-108
99-115
Average (%)
98
102
105
1:4
Range (%)
93-108
95-111
96-107
Average (%)
100
103
102
1:8
Range (%)
95-110
97-111
93-108
Average (%)
101
104
101
1:16
Range (%)
87-104
102-115
89-102
Average (%)
95
108
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
90-105
98
Cell culture media (n=5)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
201.49
406.28
1882.24
201.13
427.96
1976.55
Standard deviation
19.85
39.65
214.95
19.21
36.68
151.6
C V (%)
9.85
9.76
11.42
9.55
8.57
7.67
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HLA-B27 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HLA-B27 in samples. No significant cross-reactivity or interference between Human HLA-B27 and analogues was observed.
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