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Human Histone Deacetylase 9 (HDAC9) ELISA Kit

SKU:
HUDL01320
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9UKV0
Sensitivity:
0.138ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
HD7,HDAC,HDAC7,HDAC7B,HDAC9B,HDAC9FL,HDRP,MITR,Histone deacetylase-related protein,MEF2-interacting transcription repressor MITR,Histone deacetylase 7B
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Histone Deacetylase 9 (HDAC9) ELISA Kit

The Human Histone Deacetylase 9 (HDAC9) ELISA Kit is specifically designed for the precise quantification of HDAC9 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.HDAC9 is a key enzyme that plays a crucial role in regulating gene expression through histone deacetylation. Dysregulation of HDAC9 has been implicated in various diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions.

As such, accurate measurement of HDAC9 levels can provide valuable insights into disease pathogenesis and help in the development of targeted therapies.With its high-quality components and user-friendly protocol, the Human Histone Deacetylase 9 (HDAC9) ELISA Kit is a valuable tool for researchers looking to investigate the role of HDAC9 in disease mechanisms and explore its potential as a therapeutic target.

Product Name:Human Histone Deacetylase 9 (HDAC9) ELISA Kit
Product Code:HUDL01320
Size:96 Assays
Target:HDAC9
Synonyms:HD7, HDAC, HDAC7, HDAC7B, HDAC9B, HDAC9FL, HDRP, MITR, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR, Histone deacetylase 7B
Detection Method:Competition
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.Aspirate and wash 3 times
4.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.Aspirate and wash 5 times
6.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.Add 50µL Stop Solution. Read at 450 nm immediately.

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