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Human Histamine Receptor H4 (HRH4) ELISA Kit

SKU:
HUDL01364
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9H3N8
Sensitivity:
0.052ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
GPCR105,GPRv53,H4R,HH4R,H4,AXOR35,BG26,G-protein coupled receptor 105
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Histamine Receptor H4 (HRH4) ELISA Kit

The Human Histamine Receptor H4 (HRH4) ELISA Kit is specifically designed for the accurate measurement of HRH4 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results, making it perfect for various research applications.HRH4 is a key receptor involved in mediating the effects of histamine, a crucial mediator of the immune response and inflammation. Dysregulation of HRH4 signaling has been implicated in various inflammatory and allergic conditions, making it a valuable biomarker for studying these diseases and potential therapeutic targets.

Overall, the Human Histamine Receptor H4 (HRH4) ELISA Kit provides researchers with a reliable tool for investigating the role of HRH4 in health and disease, offering valuable insights into potential treatment strategies for inflammatory and allergic disorders.

Product Name:Human Histamine Receptor H4 (HRH4) ELISA Kit
Product Code:HUDL01364
Size:96 Assays
Target:HRH4
Synonyms:GPCR105, GPRv53, H4R, HH4R, H4, AXOR35, BG26, G-protein coupled receptor 105
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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