The Human High-Sensitive CRP ELISA Kit is a powerful tool for measuring C-reactive protein (CRP) levels in human samples such as serum and plasma with high sensitivity and accuracy. CRP is a key marker of inflammation and is commonly used in clinical settings to assess and monitor various inflammatory conditions.This ELISA kit is designed to provide precise and reproducible results, making it suitable for a wide range of research applications including studies on cardiovascular diseases, infections, autoimmune disorders, and more.
With its reliable performance and easy-to-use format, the Human High-Sensitive CRP ELISA Kit is an essential tool for researchers and healthcare professionals alike.
Product Name:
Human High Sensitive CRP ELISA Kit
SKU:
HUES03393
Target:
Human High Sensitive CRP
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human hs-CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human hs-CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human hs-CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human hs-CRP. You can calculate the concentration of Human hs-CRP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
95-111
93-109
Average (%)
103
103
100
1:4
Range (%)
89-103
80-94
86-102
Average (%)
94
87
93
1:8
Range (%)
92-105
82-93
86-99
Average (%)
97
88
91
1:16
Range (%)
90-104
83-96
81-93
Average (%)
95
88
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-97
90
EDTA plasma (n=5)
89-99
94
Cell culture media (n=5)
94-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.52
154.68
451.79
45.99
149.25
455.2
Standard deviation
3.24
7.55
24.26
2.99
8.42
19.71
C V (%)
6.68
4.88
5.37
6.5
5.64
4.33
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human hs-CRP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human hs-CRP in samples. No significant cross-reactivity or interference between Human hs-CRP and analogues was observed.
