Human HIF-1 alpha (Hypoxia Inducible Factor 1 Alpha) CLIA Kit (HUES00733)
- SKU:
- HUES00733
- Product Type:
- ELISA Kit
- ELISA Type:
- CLIA Kit
- Size:
- 96 Assays
- Sensitivity:
- 18.75pg/mL
- Range:
- 31.25-2000pg/mL
- ELISA Type:
- Sandwich
- Synonyms:
- HIF1A, HIF-1A, MOP1, PASD8, bHLHe78, basic helix-loop-helix transcription factor
- Reactivity:
- Human
- Sample Type:
- Serum, plasma and other biological fluids
- Research Area:
- Epigenetics and Nuclear Signaling
Description
Product Name: | Human HIF-1 alpha (Hypoxia Inducible Factor 1 Alpha) CLIA Kit |
SKU: | HUES00733 |
Target: | Human HIF-1 alpha (Hypoxia Inducible Factor 1 Alpha) |
Size: | 96T |
Assay type: | Sandwich |
Assay time: | 4.5h |
Sensitivity: | 18.75 pg/mL |
Detection range: | 31.25-2000 pg/mL |
Kit component: |
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This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HIF-1α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HIF-1α. You can calculate the concentration of Human HIF-1α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity: |
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Recovery: |
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Precision: |
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Sample type &Sample volume: | Tissue homogenates,cell lysates and other biological fluids; 100μL |
Reproducibility: | Both intra-CV and inter-CV are < 15%. |
Application: | This CLIA kit applies to the in vitro quantitative determination of Human HIF-1α concentrations in Tissue homogenates,cell lysates and other biological fluids. |
Specificity: | This kit recognizes Human HIF-1α in samples. No significant cross-reactivity or interference between Human HIF-1α and analogues was observed. |